Acute respiratory tract infections (ARTIs) such as bronchitis, sinusitis, and rhinitis account for almost 10% of ambulatory care visits in the United States.1 Despite having similar symptoms, upper and lower respiratory tract infections are caused by a wide range of microbes, including RNA and DNA viruses, bacteria, and fungi. However, detection of these pathogens can often be difficult. Immunoassays are limited to a small number of respiratory pathogens and lack sensitivity. While molecular detection is more sensitive, most commercially available tests are expensive, primarily focus on either viruses or bacteria, and lack the flexibility to customize target lists based on laboratory needs. In addition, concurrent prevalence of viral and bacterial pathogens is a growing concern and needs effective detection methods.